b'More data.Real-time PCR dataDeeper insights. Less effort. PRECISIONAccurate discrimination of fold changes is an important parameter for qPCR performance. To demonstrate this characteristic with the X9 System, a 1.5-fold dilution series of synthetic cDNA was prepared, and 3 EXPRESS SCRIPTS replicates of each dilution point run against 24 assays on a 192.24 Dynamic Array IFC. Amplification curves for the assay targeting GAPDH demonstrate accurate and precise quantitation of target abundance. The X9 platform supports Express scripts with which customers can reduce their genotyping and gene expression run times by 2 hours (50% less) with minimal hands-on time. This is all accomplished without changing the IFC design, requiring additional consumables and labor or impacting performance.Reduce run time by 50% withoutimpacting experiment design.Performance you can trust. High-precision performance. A 16-point dilution series of synthetic cDNA analyzed against an assay targeting GAPDH. Inset shows a NGS library preparation data magnified region of the amplification curves. REPRODUCIBILITYPERFORMANCE COMPARISON: X9 AND JUNO Uniform amplification across replicates is a requirement for accurate quantitation by qPCR. To highlight Two NGS LP 48.48 IFCs were run on the X9 System and one of its predecessors, the Juno system. Eachthe uniformity of amplification across an IFC, 96 replicates of a synthetic cDNA sample were run on the X9 IFC contained 48 samples of 125 ng of human genomic DNA. Each sample was run against 35 assaySystem against 96 assays using the 96.96 Dynamic Array IFC. A Ct standard deviation of 0.06 across 96 pools containing primer pairs to generate a total of 5,238 amplicons targeting 128 cancer genes.sample replicates on the IFC demonstrates excellent uniformity of amplification that will permit accuratequantitation of all samples, regardless of location on the IFC. X9 Juno EGFR FGFR2Number of aligned reads Number of aligned reads% On-target93.4% 93.5% Reproducible amplification. Amplification curves of 96 replicates reads of a synthetic cDNA sample run against an assay targeting GAPDH. Coverage92.4%92.0%Mean Ct is 12.4 with a standard deviation of 0.06 Ct. uniformity Amplicon Amplicon X9 Run 1X9 Run 2Juno Run 1Juno Run 2A comparison of the percent of on-targetThe number of mapped reads for representative amplicons from EGFR and FGFR2 were reads and amplicon coverage uniformitycompared for individual samples from each run. The correlation of the number of mapped demonstrates equivalent performancereads per amplicon across both systems demonstrates equivalent performance between libraries between the X9 System and thegenerated on the X9 System and Juno.Juno system. 12 13'