b'Consistency across different sample collection methods 4,5Fresh-stain In a comparison study with different experimental workflows, CyTOF demonstrates reproducible data quality 4 . For all 32 immuneStain-preservecell populations 5% in frequency, the %CV within each donorPreserve-stainacross the different workflows was 9.2%. In addition, frozenFrozen antibodyantibody cocktails are stable over time and yield comparable cocktails signal intensities over time.Fresh-stainStain-preserve workow Preserve-stain workow Read the application noteworkow Stabilizer 1 Sytodelics Stabilizer 1 SytodelicsPROT1 Ctabilizer 2 PROT1 Ctabilizer 2TBMNK cellsand granulocytesCD45+ TBMNK cells andCD45 CD123 CD123 CD3 CD8 CD45RO CD19CD66b+ granulocytes(gated on live single cells)CD66bFigure 5. Representative dot plots show the compatibility of the Human Broad Immune Profiling CyTOF Panel, 20 Antibodies with stabilization reagents and sample multiplexing in three different workflows 3 . BasophilsCD123+CD38+ basophils(gated on CD45+CD3CD19CD14CD56CD11cHLA-DR cells)CD38CASE STUDYDendritic cellsCD11c+ DCs and CD123+ Improving immunophenotyping strategies for longitudinal clinical trialsplasmacytoid DCs(gated on CD45+CD3CD19CD14CD56 12HLA-DR+ cells) Researchers at the University of Sydneydeveloped a robust CyTOF workflow to overcome the CD11climitations in remote sample collection quality, aiming to better represent more diverse populationsin clinical trials.T cells and B cellsCD3+ T cells and CD19+ B cells(gated on live singleCD45+CD66b cells) CD19 Perth Melbourne Sydney (Charles Perkins Centre)About 40 minutesCD4+ and CD8+ T cells(gated on CD45+CD19CD3+ cells) 1 2 3 4 5 6 7CD4Collect freshStain withFreeze and store. Ship to centralBarcode and stainAcquire onAutomated whole bloodready-to-useprocessing sitesamples withCyTOF system. reportCD4+ naive andsamples. custom lyophilized(Charles Perkinsfrozen CyTOF central memory T cells CyTOF panel. Centre). panel (intracellular CD45RA+ naive and CD45RO+targets).central memory T cells(gated on CD45+CD19CD3+CD4+CD27+ cells) Figure 6. This graphic highlights a highly reproducible and robust stain-freeze-ship CyTOF workflow with a 50+ functional and phenotypic panel CD45RAto capture pre- and post-treatment responses, across multiple sites. Further, they employed sample barcoding, a unique CyTOF advantage, which provides even greater data consistency for large studies.Naive and memory B cellsCD27 naive and CD27+memory B cells(gated on CD45+CD19CD3+CD4+CD27+ cells) CD27'