b'Rapid and scalable immune profilingCyTOF facilitates standardized and large-scale immunophenotyping initiativesMass cytometry analyzes immune responses at a scale not possible with other methods.The unique CyTOF advantage of discrete mass signals without overlap, and subsequent large number of available antibodies, simplifies panel design and eliminates the need for complicated spectral unmixing algorithms. Further, this wider range of available channels provides the unmatched capability for multiplexed sample barcoding, a powerful method for harmonizing large clinical trial datasets. By staining and acquiring large numbers of samples in a single tube, sample barcoding significantly reduces workflow burdens, acquisition times and technical variability, while conserving precious clinical material.CyTOF XT PRO expedites the end-to-end workflow and provides greater data consistency for large immune profiling studies. WORKFLOW COMPARISONCyTOFTime required for 30-plus markers is less than 12 weeks. In CyTOF, studies, sample preparation is simple. Design andThere are no complicated algorithms to apply to optimization Load FCS file into analysis tool. deconvolute or compensate for spectral overlap. Acquisition89Y 110Pd 141Pr 150Nd 161Dy 191lr 209BiY (1) Cd (7) Nd (7) Gd (4) Ho (1)Yb (5) Pt (4)VS Pd (6) I (1)Sm (4) Tb (1) Er (4)Lu (1) Bi (1)Rh (1) Pr (1) Eu (2)Dy (4)Tm (1) Ir (2)Spectral flowTime required for 30-plus markers is 812 weeks or more. In spectral flow, there are strict requirements for fluorophore combinations that need rigorous Design and optimization testing and optimization, single- stain controls, and additional sample processing and algorithms 7,8,9 .Manual unmixing of FCS files AcquisitionCheck 28 x 28 plots (784 plots) for signal spillover Data QCAdjust signal matrixCheck N x N plots for signal spillover; repeat as requiredLoad FCS file into analysis tool'