mRNA Sequencing 

Researchers are analyzing the transcriptome at greater resolution to uncover new mechanisms of cell development, metabolism and disease. Single-cell mRNA sequencing on the C1™ system can reveal the unique cellular makeup of tissues, including the identification of important, rare cell populations that cannot be identified by bulk analysis. This tool can also uncover the genetic programs that drive development and the mechanisms underlying cellular responses to the environment, disease and drug response. 

With the C1 single-cell mRNA sequencing workflow, you can: 

• Automatically process up to 800 individual cells per run 

• Characterize the cellular makeup of a tissue and compare the abundance of specific cell types among samples 

• Measure expression levels of genes, alleles and spliced variants 

• Compare tissue or cell type expression levels 

• Fully characterize T cell clonotypes 

• Map transcription initiation sites 

• Characterize alternate splicing patterns 

• Evaluate post-transcriptional activity 

• Discover new transcripts and gene fusions 

miRNA Expression Profiling 

MicroRNAs (miRNAs) regulate mRNA expression by mRNA degradation and gene silencing. These small, noncoding RNAs regulate thousands of human protein-coding genes that are involved in biological processes—such as differentiation, development and cell cycle management—as well as illnesses including cancer and cardiovascular disease. 

A single miRNA may regulate several biological systems, introducing cell-to-cell variability and making it difficult to reliably interpret and predict miRNA targets. Studying miRNA expression at the single-cell level offers greater insight into the differences between cells in the same population. 

Using our C1 and Biomark HD systems, the workflow for single-cell miRNA expression profiling provides an easy and reliable way to automatically capture and process individual live cells for routine miRNA analysis. 

Single-cell miRNA expression profiling allows users to: 

• Use reliable chemistry and proprietary microfluidic technology to enable cell isolation and detection of miRNAs at the single-cell level 

• Automate cell capture, staining, lysis, reverse transcription and miRNA amplification to simplify cell handling and eliminate systematic error 

• Visualize and verify single cells with a variety of surface stains 

• Generate and store miRNA libraries and interrogate single cells later for a diverse range of miRNAs 

• Go from cell to analyzed data in less than 13 hours 

• Process 96 single cells across more than 370 miRNAs in a single run 

Whole Exome Sequencing 

Exome sequencing enables the study of protein-coding regions of all 22,000 genes that are known to contain more than 85% of disease-causing variants. Bulk methods are unable to provide the clonal context of infrequent somatic mutations, making it impossible to define genetic signatures for particular cell populations. By sequencing the exome of individual cells, users can reveal the true heterogeneity and cell lineages present in a sample.

The C1 single-cell whole exome sequencing workflow is the easiest method for discovering putative variants at the first stages of your research. One integrated workflow lets users isolate, stain, lyse, amplify and prepare NGS libraries from 96 individual cells in under 24 hours. 

The C1 single-cell whole exome sequencing workflow: 

• Identifies novel or low-frequency variants at the first stage of experimental approach 

• Provides a sensitive assay and an easy workflow to individualize and process low quantities of genomic DNA from single cells 

• Collects data three times faster 

• Offers optimized protocols that reduce standard reagent costs

Single-Cell Proteomics 

Protein Analysis 

Understanding complex processes in heterogeneous tissues lies at the heart of modern biology. The ability to identify cell subpopulations and their state and to characterize their function is leading to breakthroughs in basic analysis, translational research and drug development. 

Single-cell protein analysis by mass cytometry enables the most comprehensive profiling of cell surface and intracellular protein markers. With Maxpar® metal-conjugated antibodies, a system and optimized data analysis and visualization tools, you can perform single-tube mass cytometry experiments to produce data on: 

• Phenotype 

• Signaling state 

• Transcription factors 

• Cytokine, chemokine and growth factor expression 

• Proliferation and cell cycle status 

• Health and viability 

Make the complex routine. Learn more about CyTOF® mass cytometry.