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Immune profiling

Reveal the immune features driving biological change

The complexity of the immune system demands high-dimensional analysis. Easily characterize breadth and depth of immune cell subpopulations with CyTOF™ panels to gain deeper insights into complex biology.

 

Simple and standardized immune profiling

Immune profiling identifies and quantifies immune populations according to their phenotypic and functional features. It enables researchers to broadly understand unique immunologic response to disease or therapeutic interventions, and can potentially predict health outcomes.

Improve longitudinal and multi-site studies with the option to preserve and store samples as needed – enabling more flexibility to stain and process samples at any time and from any location, with CyTOF immune profiling.

Flexible options to bring immune profiling to your research

Choose from the preconfigured and validated CyTOF panels below or let SBI Lab Services support your project from sample to data.

Maxpar Direct Immune Profiling Assay

A complete sample-to-answer solution for single-cell, high-dimensional immune profiling of human PBMC and whole blood with proven performance and reproducibility

The Maxpar™ Direct Immune Profiling Assay and Maxpar Pathsetter software provide an easy-to-implement single-tube assay for routine and reproducible sample collection to capture the highly individual nature of immune response.

  • Identify 37 circulating immune cell types in human whole blood or PBMC samples with the validated, dry-format assay
  • Standardize immune monitoring studies in a single-tube workflow with automated reporting and data analysis
  • Minimize variability and ensure high reproducibility of multiparameter data

Available in two sizes, a 25-test kit and 5-test kit format.

View product brochure →

 

Designed for clinical research

Variability in sample handling, panel preparation, and data analysis can make it difficult to compare results across sites or over the course of a study. MDIPA’s standardized workflow makes it ideal for clinical trials where reproducibility is critical.

MDIPA set the benchmark for standardized immune profiling and is:

  • Used in hundreds of studies and many clinical trials
  • <10% CV between sites and instruments
  • Easily expanded with add-on panels or markers of interest
  • Compatible with stabilizing reagents, stain-freeze workflows and barcoding methods

 

The reproducibility of the Maxpar Direct Immune Profiling System has been demonstrated run-to-run and site-to-site, ideal when samples are processed and analyzed by different technicians or at different study sites.

 

Learn how this single-tube assay speeds up sample collection to insights

 

Easily expand and customize with Maxpar Direct Expansion Panels

Easily build on the Maxpar Direct backbone panel with individual antibodies or Expansion Panels, designed to enable deeper profiling of specific immune cell subsets that are relevant in immune-mediated disease, such as infectious disease, cancer, autoimmunity or allergy.

Choose from 9 Expansion Panels
  • Surface profiles
  • Cell proliferation
  • Cell exhaustion
  • Apoptosis
  • Metabolism
  • Phosphoproteins
  • Cytokine production
  • Transcription factors

Explore expansion panels

More flexibility with a single-tube assay

A ready-to-use assay that eliminates variability and reduces errors from liquid pipetting and panel preparation.

CyTOF immune profiling panels are fully compatible with popular stabilization reagents, conserving cell population integrity even under fixed conditions. Whole blood samples can be preserved at any time point, eliminating time-sensitive challenges when processing samples for multi-site and longitudinal studies.

Application note: A 20-Marker Core Immune Flow Cytometry Panel Optimized for Flexible Whole Blood Preservation Workflows 

5-minute reporting

Maxpar™ Pathsetter is a fully automated reporting and data analysis solution that automatically identifies 37 immune cell types in FCS files from samples processed with the Maxpar Direct Immune Profiling System.

  • Automated selection and enumeration of single viable cells
  • A high-dimensional Cen-se′ (next-gen t-SNE) map of identified populations
  • A report summary for each population, with cell number, percent of total cells and percent of parent population
  • A QC metrics report for acquisition and modeling quality

Pathsetter page

Did you know you can create your own lyophilized panel?

Advancing on the validated dry-format Maxpar™ Direct Immune Profiling Assay, custom lyophilized LyoMax™ Panels provide the same streamlined workflow but are individually tailored to specific markers of interest and standardized for large batches.

Take a look

Key publications showcasing lyophilized immune profiling panels

 

Featured publication: Deep phenomics of PPR agonist activated human blood, Barman et al. 2025 – The study shows that differences in cellular composition can substantially alter the immune signatures observed in vitro, highlighting the importance of selecting the appropriate sample type for immune profiling.


Flex-Fit CyTOF Panels

Bringing versatility to panel design

Build your own panel with ready-to-go pre-validated CyTOF modular panels, which overcome common challenges, complexity and labor typically involved with flow cytometry panel design. Uncover biomarkers of immune potency and inflammatory response by easily profiling immune cell type and function – in a single panel.

View product brochure →

Mix and match CyTOF panels reduce time of study design and optimization

  • Reveal functional diversity of immune checkpoint markers and cytokines
  • Resolve rare cell populations with exceptional resolution to uncover true functional biology
  • Optimized for fresh or fixed whole blood and PBMC samples to capture key markers of interest

 

How it works: Build an immune profiling backbone for any study

 

Start with the essentials

Choose the Human TBMNK+G CyTOF Panel, 9 Antibodies to identify major immune cell subsets (T cells, B cells, myeloid cells, NK cells and granulocytes) or the Human Broad Immune Profiling CyTOF Panel, 20 Antibodies (20 markers identifying 32 immune cell types).

 

Go beyond

For greater coverage of immune cell subsets, add any modular Flex-Fit CyTOF panel or set of panels. And then add additional customizations with individual markers of your choice, if desired. Pre-selected bundles are available. Learn more →

 

Easily customize panels by adding or replacing markers of interest
With more than 30 open channels, you are ready to add more targets of interest.
Discover our collection of CyTOF catalog antibodies to target your research objectives.

Two example high-parameter bundles built from Flex-Fit CyTOF Panels allow for comprehensive characterization of cell
populations and functional dynamics

 

These 2 bundles can be further expanded or customized by adding antibodies in the available open channels to address a variety of research questions.

Panel details

Human TBMNK+G CyTOF Panel, 9 Antibodies

Human Broad Immune Profiling CyTOF Panel, 20 Antibodies

Part numbers end in B for 100 µL test size and C for 25 µL test size.


Maxpar OnDemand Mouse Immune Profiling Panel Kit

Simplifying high-parameter cytometry for mouse studies, from end to end

The Maxpar OnDemand Mouse Immune Profiling Panel Kit includes everything required to stain and analyze your mouse samples. Maxpar Pathsetter templates are available to expedite analysis from data to insights.

An end-to-end solution for high-parameter mouse studies.

Designed as a simple workflow, the solution brings together CyTOF technology, a 33-antibody reagent kit and Maxpar Pathsetter templates, enabling identification of 38 mouse immune cell populations. Maximize insights for preclinical and discovery research.s

Here’s an example of how the Maxpar OnDemand Mouse Immune Profiling Panel Kit enables the characterization of 38 splenic immune subsets in Maxpar Pathsetter analysis.

Available resources for download:

Simple customization and panel expansion

Easily customize panels by adding or replacing markers of interest. With 15 open channels, you are ready to add more targets of interest.

Adding 10 Maxpar antibodies to the Maxpar OnDemand Mouse Immune Profiling Panel Kit enabled characterization of 55 pulmonary immune subsets with automated Pathsetter analysis. Take a look at the 66 new metal-tagged antibodies for mouse studies.

 

Product details

The Maxpar OnDemand Mouse Immune Profiling Panel Kit includes:
  • 33 surface antibodies (25 µL each, approximately 25 tests)
  • All buffers (Cell Staining, Fix and Perm, Cell Acquisition) Rh and Ir

Ordering information

Resources

CyTOF panel flyer

Human TBMNK+G CyTOF Panel

Human Broad Immune Profiling CyTOF Panel

Technical note: Assay Validation Processes to Ensure Accuracy and Precision in Immune Profiling – Advancing Single-Cell Proteomic Applications

Mass cytometry panel application note

High-parameter cytometry flyer

Don’t have a CyTOF system? We can help!

Simply stain and ship samples to us. Get raw and analyzed data results within 72 hours.

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