Mass Cytometry
Driving groundbreaking insights at the single-cell level, millions of times
Mass cytometry, powered by CyTOF® technology, is the world’s most advanced cytometric platform. It has opened new doors to discovery and clinical research by empowering investigators to interrogate more than 50 markers simultaneously on millions of individual cells. Join the hundreds who have reimagined cytometry with CyTOF to unveil new cell types, functions and biomarkers in immunology, cancer, infectious disease and more.
Workflow
1
Design panel
Easily build panels with up to 50 metal-tagged antibodies and reagents using Maxpar® Panel Designer. Add barcoding to multiplex your samples for even better data quality. Or use pre-built panels to get started!
2
Prepare samples
CyTOF systems provide highly multiplexed single-cell data from cell suspension samples. Standard protocols for preparing single-cell suspensions from solid or liquid tissues and tumors can be used for CyTOF sample prep. Check out our support page for staining protocols.
3
Acquire data
The CyTOF XT™ system has fully automated pellet resuspension, calibration bead addition and acquisition of sample batches from the Autosampler carousel.
The Helios™ system provides the same excellent standard of data quality as CyTOF XT but requires manual resuspension and loading of samples.
4
Analyze data
CyTOF systems produce standard FCS 3.0 (Helios) or 3.1 (CyTOF XT) data files that can be analyzed with any compatible software, including FCS Express™ and Maxpar Pathsetter™.
TRENDING NOW PUBLICATIONS TOPICS
Explore these compilations of recent mass cytometry publications focused on specific areas of investigation.
Prevention and treatment of neurological disease
Vaccine development and efficacy testing
CyTOF for Drug Development
CyTOF for CAR Therapy
Maxpar Direct Immune Profiling Assay
COVID-19 research update
CAR T cells
The value of high-parameter cytometry
Immuno-oncology
Cancer vaccine development
Vaccines for infectious disease
COVID-19 disease research
APPLICATIONS
WEBINARS
Jennifer Snyder-Cappione, PhD
Assistant Professor of Virology,
Immunology & Microbiology
Boston University School of Medicine
Overcome the challenges of functional profiling in spectral flow cytometry – with CyTOF XT.
A must-see session highlighting what could be missing in spectral flow cytometry results. A head-to-head comparison of intracellular staining panels using a spectral flow cytometer and the CyTOF XT™ mass cytometer.
Watch more mass cytometry videos and webinars.
Single-Cell Immune Profiling Can Help Resolve Tumor Microenvironment Complexities | GEN
Katie Vowell, PhD
Principal Investigator – Immunology, GlaxoSmithKline
Mass Cytometry for Precision Medicine | Petter Brodin, MD, PhD
Petter Brodin, MD, PhD
Associate Professor of Immunology, Imperial College London, Karolinska Institute
Director, SciLifeLab Mass Cytometry Facility
Bring efficiency and reproducibility to biomarker discovery and immunophenotyping studies: CyTOF XT™
Philip Hobson, PhD
Deputy Manager, Flow Cytometry Science and Technology Platform, The Francis Crick Institute
Events
Join us for one of our upcoming events or watch on demand.
VIEW MASS CYTOMETRY EVENTS
VIEW ALL EVENTS
Nov 18 - Nov 20
Location
SifrARN (Structure, Integration, Function and Reactivity of RNA) in Lyon, France
Nov 21 - Nov 22
Location
Spatial Biology Congress Asia in Singapore
Nov 28 - Nov 30
Location
ACC (Alleanza Contro il Cancro) in Reggio Emilia, Italy
Dec 01 - Dec 03
Location
Multi-Omics 2024 in Brisbane, Australia
Dec 03 - Dec 05
Location
The 53rd Academic Conference of the Japanese Society for Immunology in Nagasaki, Japan
Dec 03 - Dec 04
Location
HCA Asia (Human Cell Atlas) in Hong Kong
Dec 03 - Dec 05
Location
Precision Medicine and the Future of Genomics in Doha, Qatar
Dec 04 - Dec 05
Location
Spatial Biology West Coast in San Diego, CA
Dec 04 - Dec 08
Location
ASI (Australian and New Zealand Society for Immunology) in Auckland, New Zealand
Dec 06 - Dec 08
Location
Pan-IIT Meeting & Conference on Engineering in Medicine in Kanpur, India
Resources
Bibliographies
Flyers & brochures
Spotlight articles
Technical documents
User guides
Tools
No CyTOF? No problem!
We can help you convert your fluorescence panel and find a service provider.
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.