Sample Identification
Sample identity and the integrity of genomic data are critical for execution of superior research. Particularly with complex multiphase studies, aliquots from a single specimen may be shared among researchers within a laboratory or with external collaborators and service providers.
It is a universally recognized priority to ensure accurate sample tracking and quality acquisition, all done through data reporting. This methodology can extend from high-throughput genomics centers to biorepositories or centralized cell line banks.
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Confidently track samples and assess quality using automated microfluidics
Biobanks and genomics centers must overcome several challenges to ensure that they are providing the highest quality and the correct samples to research projects.
Sample mix-ups, contamination or handling errors can occur before or after samples enter the molecular laboratory or storage facility.
Implementing a standard genotyping workflow to confirm the identity and quality of each sample before analysis can maximize the integrity of study results.
Processing misidentified, poor-quality or contaminated samples may lead to incorrect interpretation of results.
The Advanta™ Sample ID Genotyping Panel provides a high-throughput and automated solution for molecular sample identification, enabling you to simultaneously perform molecular fingerprinting, quality assessment and integrity confirmation on every sample. The panel can be used with multiple genomic workflows and is customizable for any sample type.
Sample mix-ups, contamination or handling errors can occur before or after samples enter the molecular laboratory or storage facility.
Processing misidentified, poor-quality or contaminated samples may lead to incorrect interpretation of results.
molecular sample identification
What is molecular sample identification?
This proactive sample identification system is also known as sample ID or DNA fingerprinting. Panels of DNA markers are analyzed for each sample, generating a unique genotype or fingerprint. The genotype obtained is an indelible, nontransferable identifier for the sample.
Carefully selected markers can provide additional information, such as:
- Assessment of sample quality
- Genetic gender identity
- Population prediction
How is it used?
DNA samples can be fingerprinted (identified) prior to distribution.
Sample identity can be confirmed.
- Compare genetic sex to reported sex.
- Compare genotypes from distribution sample to accession genotypes.
Low-quality DNA issues can be identified.
- Low genotype calling rate
- Sample contamination
Advanta Sample ID workflow
Microfluidics-based fingerprinting provides outstanding reproducibility with automated reaction mix assembly, which enables high-throughput processing of up to 9,216 results per run. The nanoliter reaction volume requirements reduce reagent cost and maximize sample usage.
The workflow allows for:
- Maximum targets in individual reactions with no multiplexing
- Direct compatibility with common probe-based assays
- Flexibility with no pre-spotted assays
Webinars
Safeguarding Biobank Sample Integrity with High-throughput Molecular Fingerprinting
A presentation by Luke Stewart, Michael Denton, and Molly Walling
Experts from the Indiana University Genetics Biobank (IUGB) and Standard BioTools discuss how SNP fingerprinting workflows on the Biomark™ system support high-confidence sample identity and quality control in biobanking. Discover how molecular fingerprinting can safeguard against mishandling of specimens, streamline quality control processes and scale across large collections. This session covers assay design considerations, operational best practices and lessons learned from implementing automated, high-throughput identity verification in an established biorepository.
From high-throughput genomics centers to biorepositories or centralized cell line banks, ensuring accurate sample tracking and quality from acquisition through data reporting is a universally recognized priority.
Hear Luke Stewart, Senior Director of Microfluidics, detail the Advanta Sample ID Genotyping Panel, a 96-SNP genotyping assay that enables sample identification and quality assessment. Leveraging microfluidics technology, the Advanta Sample ID workflow offers a number of benefits including decreased hands-on time and significant cost savings compared with traditional qPCR methods.
customer stories
Learn more about how our customers are leveraging Standard BioTools technology
Kelly Nudelman, PhD
Kelly Nudelman and Tae-Hwi Schwantes-An discuss sample identification
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