Gene Expression

Advance your translational and clinical research with TaqMan or Delta Gene assays

Data reproducibility is key to differential gene expression studies. The scalable Biomark™ platform supports tens to hundreds of samples at nanoliter volumes. Combining microfluidics technology with optimized reagents produces high-quality results in less time. Ideal for labs performing hundreds to thousands of samples, the Biomark X9 system provides significant cost and time advantages over plate-based technologies. And with a wide range of integrated fluidic circuit (IFC) formats available, scaling from mid- to high-throughput on a single system is easily achievable.

Methods

Delta Gene

Pair Delta Gene™ assays and reagents with EvaGreen dye for an economical alternative to probe-based detection. EvaGreen binds to double-stranded DNA, resulting in fluorescein-like fluorescence excitation and emission spectra. EvaGreen also minimizes PCR inhibition compared with SYBR Green and other nucleic acid stains.

TaqMan

Probe-based assays use primers to detect sequence-specific single-cell cDNA probes. The method enhances qPCR through dual-labeled hydrolysis probes and PCR primers. Specific target detection requires binding three independent oligonucleotides, which reduces fluorescence from nonspecific sources. Our chemistries work seamlessly with your existing TaqMan assay library to support both standard and fast protocols.