Methods
SNP Type Genotyping
SNP Type assays consist of unlabeled allele-specific primers and universal probes, making them an affordable alternative to probe-based genotyping assays. Lower price points make it possible to design and test for multiple markers and empirically choose the best. Select optimal assays designed for a variety of species using your supplied sequences. Optimized SNP Type preamplification protocols boost results from poor-quality, low-concentration or precious samples.
TaqMan Genotyping
TaqMan probe-based SNP genotyping relies on dual-labeled probes and PCR primers. Each assay shares a pair of primers flanking the SNP and two or more allele-specific cleavage probes. Proximity suppresses fluorescent signaling until the probe specifically binds to the target. During PCR nuclease activity degrades the probe, resulting in increased brightness. Our reagents support TaqMan assays run on Biomark, EP1™ and Juno™ platforms.
Customer Stories
Learn more about how our customers are leveraging Standard BioTools™ technology
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To singleplex or multiplex? What’s the difference? →
These two detection methods involve different assay design and setup, and each one has its own set of advantages and disadvantages.