CyTOF™ XT PRO
Workflow and applications
Learn more about the CyTOF XT PRO specifications
Applications
Revealing predictive proteomic insights
Robust immunophenotyping strategies for drug discovery and development are crucial to better understand the immune response to disease and therapeutics. The challenge is capturing a wide enough range of cell types and their functions to account for biological heterogeneity –essential for identifying predictive biomarkers, stratifying patients and understanding mechanisms of action. High-dimensional panels comprised of intracellular and surface markers are ideal for clinical cohorts, as they increase the amount of information acquired from limited precious samples. For this reason, CyTOF technology has been adopted for use in over a hundred clinical trials worldwide for immune monitoring and biomarker discovery studies, as it enables researchers to reliably capture 50-plus targets simultaneously, across areas including immuno-oncology, infectious disease, autoimmune disease and neuroscience.
The top cancer immunotherapies have leveraged unique biomarkers found by CyTOF technology.
This application note highlights case studies leveraging CyTOF technology to reveal mechanism of action, predictive response and relevant biomarkers across a variety of therapeutic areas and indications better than other cytometric technologies.
Therapeutic applications
Workflow
Consistency across different sample collection methods, for both PBMC and whole blood, gives clinical researchers flexibility in study design and the ability to scale larger and broader cohorts with versatile workflow options, including:
Fresh-stain
Stain-preserve
Preserve-stain
Frozen antibody cocktails
Representative dot plots show the compatibility of the Human Broad Immune Profiling CyTOF Panel, 20 Antibodies with stabilization reagents and sample multiplexing in three different workflows.
CyTOF technology has exceptionally high reproducibility, as metal-tagged antibodies can withstand the harsh elements of time and temperature. In a comparison study with different experimental workflows, CyTOF technology demonstrates reproducible data quality. For all 32 immune cell populations ≥5% in frequency, the %CV within each donor across the different workflows was <9.2%. In addition, frozen antibody cocktails are stable and yield comparable signal intensities over time.
Read the application note.
An easy-to-implement solution for routine and reproducible immune profiling
This video highlights a streamlined and standardized workflow for the Maxpar™ Direct Immune Profiling Assay, a dry-format 30-marker antibody panel optimized for deep phenotyping of human peripheral whole blood and PBMC. This robust assay combines a single-tube workflow with an automated five-minute data analysis solution to quickly phenotype 37 immune cell populations, with no titrations needed. It provides researchers with a routine and reproducible method to better understand the variation in immune response to disease pathogenesis and therapeutic interventions.
Technology
Cytometry by time-of-flight or mass cytometry (on which CyTOF technology is based) is a proteomic technology that uses metal-conjugated antibodies to simultaneously analyze 50-plus intracellular and surface markers in biological samples at single-cell resolution. This overcomes the limitations of spectral overlap and post-stain sample instability associated with fluorescence flow cytometry, enabling greater immune coverage, higher reproducibility and consistency, and faster execution of large immunophenotyping studies.
Learn more about mass cytometry
Uncovering diverse and rare immune cell subsets, and their functions, with CyTOF technology
Hear why researchers are choosing CyTOF technology for their immune profiling studies. Between data accuracy and simple panel design, CyTOF users explain why it is the only technology capable of measuring 50-plus parameters to drive breakthroughs in immunology and cancer research.
Maxpar Direct Immune Profiling Assay 30 Marker—25 Tests
PN 201334 replaces PN 201325. The Maxpar® Direct™ Immune Profiling Assay™ is a dry 30-marker antibody panel designed and optimized for deep immune profiling of human peripheral whole blood and PBMC. The assay enables comprehensive identification and characterization of 37 immune cell populations.…
Cell-ID™ 20-Plex Pd Barcoding Kit
The kit enables unique barcoding of 20 samples so they can be combined and subsequently stained and acquired as one multiplexed sample, followed by software debarcoding and individual sample analysis. Multiplexing samples improves data quality, since the 20 samples are stained, processed, and acquired as one sample, eliminating sample-specific staining and data collectio…
Maxpar® Cell Acquisition Solution Plus for CyTOF® XT - 1000 mL
Maxpar® Cell Acquisition Solution (CAS) Plus for CyTOF XT™ is an improved high ionic strength solution intended for sample preparation and sample acquisition on CyTOF XT systems.
EQ™ Six Element Calibration Beads—100 mL
The 6-element EQ6 Beads solution is intended for the following uses on the CyTOF XT™ system. Maxpar® Cell Acquisition Solution (CAS) Plus for CyTOF XT™ is an improved high ionic strength solution intended for sample preparation and sample acquisition on CyTOF XT systems…
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