Conjugation Services
Maxpar Antibody Conjugation Service for Flow Cytometry and Imaging Mass Cytometry
Expand and accelerate your research with isotope-conjugated antibodies made by experts
The use of metal-labeled antibodies as part of CyTOF® technology is the most comprehensive way to profile cell phenotype and function for cells in suspension or in tissue samples, enabling simultaneous detection of over 40 cellular targets for imaging or 50 cellular targets for suspension in every precious sample.
Whether you’re building a mid-range or high-plex CyTOF panel, we offer the extensive Standard BioTools™ catalog of preconjugated antibodies and antibody panel kits to help get you there.
We understand and can help with your panel design needs. If you have a proprietary antibody to add to your panel or require customized design, our Maxpar® Antibody Conjugation Service is committed to your success.
Select the desired isotope from our 51 high-purity metal tags, decide which confirmation method you prefer – biochemical analysis or more extensive biological functional testing – and place your order.
After our expert scientists perform the conjugation and analysis, we’ll send your antibody conjugate along with a detailed analytic report.
Save time and resources
Let our experts do the labeling and quality control for you.
Flexible
Extend your mass cytometry panels by adding metal-labeled targets not yet available in the Maxpar catalog.
Expertise
Conjugation service performed by our professional technicians ensures product with consistently high-quality and reliable performance.
Custom Conjugation Workflow
Custom Conjugation Services Metal Options for Flow Cytometry and Imaging Mass Cytometry
Flow Cytometry (51 metal options)
| Metal | Isotope |
|---|---|
| Y | 89 |
| Cd | 106 |
| Cd | 110 |
| Cd | 111 |
| Cd | 112 |
| Cd | 113 |
| Cd | 114 |
| *In | 115 |
| Cd | 116 |
| *La | 139 |
| Pr | 141 |
| Nd | 142 |
| Nd | 143 |
| Nd | 144 |
| Nd | 145 |
| Nd | 146 |
| Sm | 147 |
| Metal | Isotope |
|---|---|
| Nd | 148 |
| Sm | 149 |
| Nd | 150 |
| Eu | 151 |
| Sm | 152 |
| Eu | 153 |
| Sm | 154 |
| Gd | 155 |
| Gd | 156 |
| Gd | 158 |
| Tb | 159 |
| Gd | 160 |
| Dy | 161 |
| Dy | 162 |
| Dy | 163 |
| Dy | 164 |
| Ho | 165 |
| Metal | Isotope |
|---|---|
| Er | 166 |
| Er | 167 |
| Er | 168 |
| Tm | 169 |
| Er | 170 |
| Yb | 171 |
| Yb | 172 |
| Yb | 173 |
| Yb | 174 |
| Lu | 175 |
| Yb | 176 |
| Pt | 194 |
| Pt | 195 |
| Pt | 196 |
| *Au | 197 |
| Pt | 198 |
| Bi | 209 |
Imaging Mass Cytometry (40 metal options)
| Metal | Isotope |
|---|---|
| *Y | 89 |
| *In | 115 |
| Pr | 141 |
| Nd | 142 |
| Nd | 143 |
| Nd | 144 |
| Nd | 145 |
| Nd | 146 |
| Sm | 147 |
| Metal | Isotope |
|---|---|
| Nd | 148 |
| Sm | 149 |
| Nd | 150 |
| Eu | 151 |
| Sm | 152 |
| Eu | 153 |
| Sm | 154 |
| Gd | 155 |
| Gd | 156 |
| Gd | 158 |
| Tb | 159 |
| Gd | 160 |
| Dy | 161 |
| Dy | 162 |
| Dy | 163 |
| Dy | 164 |
| Ho | 165 |
| Metal | Isotope |
|---|---|
| Er | 166 |
| Er | 167 |
| Er | 168 |
| Tm | 169 |
| Er | 170 |
| Yb | 171 |
| Yb | 172 |
| Yb | 173 |
| Yb | 174 |
| Lu | 175 |
| Yb | 176 |
| Pt | 195 |
| Pt | 196 |
| Pt | 198 |
*New metals added to the catalog are indicated by an asterisk and with yellow highlighting.
Custom Conjugation Services Specifications and Ordering Information
With Biochemical Confirmation |
With Biological Confirmation* |
||
|---|---|---|---|
| Biochemical analysis provided | ✓ | ✓ | |
| Biological confirmation provided* | — | ✓ | |
| Staining profile and titer data | — | ✓ | |
| Timeline | 3 to 4 weeks from receipt of antibody | 5 to 6 weeks from receipt of antibody | |
| Flow Cytometry Antibodies | |||
| Scale: standard Antibody input: 200 µg Buffer: purified, protein-free buffer |
PN 3999999-2 Minimum yield: 40 µg |
PN 3999999-1 Yield: 100 tests (1–3 x 106 cells) |
|
| Scale: bulk Antibody input: variable mass Buffer: purified, protein-free buffer |
PN 3999999-7 Minimum yield: 200 µg |
PN 3999999-8 Minimum yield: 600 µg |
PN 3999999-5 Yield: 100 tests (1–3 x 106 cells) Extra 100-test vial |
| Imaging Mass Cytometry™ Antibodies | |||
| Scale: standard Antibody input: 200 µg Buffer: purified, protein-free buffer |
PN 3999999-2D Minimum yield: 40 µg |
||
| Scale: bulk Antibody input: variable mass Buffer: purified, protein-free buffer |
PN 3999999-7D Minimum yield: 200 µg |
PN 3999999-8D Minimum yield: 600 µg |
|
* Not available for antibodies used in Imaging Mass Cytometry
Ligands and buffer suitable for custom conjugation – IgG monoclonal and polyclonal antibodies are routinely conjugated. The feasibility of conjugating other antibody isotypes and proteins is considered on a case-by-case basis. To facilitate labeling, ligands must be provided purified, unconjugated and in a protein-free buffer at minimum concentration of 0.5 mg/mL.
Source of antibodies – Antibodies to be custom conjugated may be either provided by the customer or purchased from a commercial source. Where Standard BioTools purchases an antibody on behalf of a customer, the cost is quoted and billed as a separate line item, PN 3999999-3, Antibody Material Handling Charge.
Quality control – All custom conjugations are subject to rigorous biochemical confirmation, including inductively coupled plasma mass spectrometry, determination of concentration and yield and assessment of ratio of attachment of metal atoms to antibody. Optionally available biological confirmation service involves empirical testing by CyTOF on cells, with optimal titer determined and the conjugate formulated for delivery in a 100-test vial.
Contact us to learn more about Maxpar Custom Antibody Conjugation Services.
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