CyTOF™ XT: The NeXT Evolution
Preview the new CyTOF XT system
TECHNOLOGY
Driving groundbreaking insights at the single-cell level, millions of times
Mass cytometry, powered by CyTOF technology, is the world’s most advanced cytometric platform. It has opened new doors to discovery and clinical research by empowering investigators to interrogate more than 50 markers simultaneously on millions of individual cells. Join the hundreds who have reimagined cytometry with CyTOF technology to unveil new cell types, functions and biomarkers in immunology, cancer, infectious disease and more.
- XTra autonomy: CyTOF XT automation expands what your lab can accomplish by simplifying daily workflows and freeing operator time for other tasks
- XTra efficiency: Simplified front-end assembly, a bottle tray for solutions and automated tuning minimize maintenance and calibration
- XTra clarity: The precision of measuring marker expression using mass cytometry is unmatched by fluorescence cytometry methods. Cleaner signals mean data is more accurate.
Overcome the challenges of functional profiling in spectral flow cytometry – with the CyTOF XT system
A must-see session highlighting what could be missing in spectral flow cytometry results. Learn more about a head-to-head comparison of intracellular staining panels using a spectral flow cytometer and the CyTOF XT mass cytometer.
Jennifer Snyder-Cappione, PhD
Assistant Professor of Virology,
Immunology & Microbiology
Boston University School of Medicine
GET MORE INFORMATION
Interested in Standard BioTools™ mass cytometry products or need a quote? Contact our sales team for product orders, quotes or other inquiries.
RESOURCES
Videos
Flyers & Brochures
Technical documents
- The CyTOF XT System: The Next Generation of Mass Cytometry Application Note | FLDM-00462
- CyTOF XT Specification Sheet | FLDM-00464
- Poster: CYTO 2021 – CyTOF XT and Helios™ mass cytometer performance comparison for the Maxpar Direct Immune Profiling Assay
- The CyTOF XT System: Advancements in Data Acquisition and Processing Technical Note | FLDM-00678
Frequently asked questions
RELATED SOFTWARE
Get the precise results your research deserves!
CyTOF Software v9.3
CyTOF™ Software v9.3 offers instrument control and data acquisition functionality for both imaging and flow applications with the Hyperion™ XTi Imaging System, CyTOF XT System and CyTOF XT PRO Syst... v9.3 September 01, 2025
Maxpar Pathsetter
Maxpar™ Pathsetter is a fully automated reporting and data analysis solution that automatically identifies 37 immune cell types in FCS files from samples processed with the Maxpar Di... v3.0.15 November 17, 2025
FCS Express
FCS Express is a fully integrated analysis, statistics, graphing and reporting tool for turning cytometry data into presentation-ready and publication-ready results. Full support for Standard... July 06, 2020
SPECIFICATIONS
| CyTOF XT System | Workstation | Data file specifications | CyTOF XT instrument dimensions |
|---|---|---|---|
| Channels 135 | CPU Intel® Core™ i7 | .fcs 4 bytes/channel/event | Width 93 cm (37 in) |
| Mass range 75–209 amu | RAM 32 GB DDR4 | .lmd Compressed format. 5–20 GB/hr (typical) | Height 135 cm (53 in) |
| Abundance sensitivity <0.3% for M+1 with M = 159Tb <0.3% for M–1 with M = 175Lu | Data storage 3.6 TB | _SPR.fcs 3.82 MB/hr | Depth 75 cm (30 in) |
| Instrument response 500 159Tb dual counts/EQ™ Six Element Calibration Beads | Monitor 86 cm (34 in) LED | _BG.fcs 0.48 MB/hr | Weight 288 kg (635 lb) |
| Dynamic range 4.5 orders of magnitude | Keyboard and mouse - Wired | _Processed.fcs 4 bytes/channel/event | |
| Calibration Automated | |||
| Operating system Windows® 10 Enterprise LTSC | |||
| Sample introduction Dual syringe drive | |||
| Flow rate 30 µL/min | |||
| Carryover <1% | |||
| Sample format 12 x 5 mL tube positions 1 x 15 mL tube positions | |||
| Reagent reservoir Maxpar® Cell Acquisition Solution Plus for CyTOF® XT 1L Maxpar Water 500 mL Maxpar Washing Solution for CyTOF XT™ 500 mL |