New! Masterclass: Unlocking Assay Design with D3
Hear Isaac Kim, Senior Assay Design Scientist at Standard BioTools, discuss how to use D3™ Assay Design, a web-based tool for creating custom gene expression, genotyping, and targeted NGS panels. Learn how the platform addresses complex research needs, such as non-model organisms or emerging pathogens, that standard off-the-shelf panels cannot meet, and see how case studies, including wildlife genomics in Germany and Finland, GMO detection in Switzerland, and neuroscience research, demonstrate the utility of custom-designed assays.
Simplify panel development with D3 Assay Design
Automated microfluidics-based workflows for genomic analysis applications help streamline laboratory methods and substantially reduce costs. With D3 Assay Design, powerful algorithms enable you to quickly define parameters for targeted NGS library prep, genotyping and gene expression assays.
Define
Define your targets.
Design
D3 designs a panel driven by user-identified parameters.
Deliver
We deliver the ideal primers for your NGS, genotyping or gene expression research.
D3 Assay Design services can help you:
Simplify the complex
Confidently develop custom PCR and NGS library prep panels with a robust design algorithm that provides optimal primer performance for your projects.
Design quickly
Use the intuitive D3 design interface to create any size panel in two days or less.
Connect to support
Easily engage with our design experts for assistance with challenging target designs and project-specific needs.
Get started with D3 Assay Design
For researchers who value the carefully selected targets included in our Advanta™ Solid Tumor NGS Library Prep Assay or Advanta Sample ID Genotyping Panel but wish to add or substitute primer pairs, the D3 assay design tool enables easy modification for your desired content.
Consider which assay type is appropriate for your project and instrument workflow:
SNP TYPE™ ASSAYS
Cost-effective single-nucleotide polymorphism (SNP) genotyping assays with performance comparable to TaqMan genotyping assays
For use on Biomark™ and EP1™ systems
TARGETED DNA-SEQ LIBRARY ASSAYS
Highly multiplexed targeted NGS sequencing primers for use with Advanta NGS Library Prep chemistry on Illumina systems
For use on Biomark and Juno™ systems
DELTA GENE™ ASSAYS
High-quality, economical real-time PCR gene expression assays with performance comparable to TaqMan gene expression assays. Assays are offered with or without wet-lab testing for human or mouse panels.
For use on Biomark systems
ACCESS ARRAY TARGET- SPECIFIC PRIMERS
Targeted NGS sequencing primers for use with Access Array™ chemistry on Illumina systems. Analvze up to 480 targets at a time with the NGS LP 48.48 IFC.
For use on Access Array systems
Follow these four easy steps:
1. Create
Create an account at d3.standardbio.com.
2. Select and define
Select your assay type and define your targets of interest. D3 Assay Design accepts a variety of targets such as gene names and user-provided sequences. Design results are sent to you within 48 hours.
3. Review and finalize
Review the design results and make any necessary modifications. Finalize your panel by requesting a quote from your sales representative.
4. Get delivered
We will manufacture and deliver your assays that work seamlessly with Standard BioTools reagents and protocols.
For additional guidance, refer to the D3 Assay Design User Guide.
The microfluidics advantage
All D3 custom assays are preformulated in 96- or 384-well plates and designed to integrate seamlessly with Standard BioTools™ microfluidics-based systems. Simply load samples and assays into the integrated fluidic circuit (IFC), then place the IFC into the instrument, where reagent mixing and chemistry proceed. IFC technology significantly minimizes pipetting steps, hands-on time and reagent consumption while maximizing efficiency and cost savings.
We’re here to help with your custom panel design needs
Our dedicated D3 Assay Design Group provides support for custom panels and is available to answer any inquiries. Contact your local sales representative or Field Applications Scientist.
Talk to an expert